V-095 | Bacterial diets are able to modulate life-history treats in C. elegans model of Parkinson disease

V-095 | Bacterial diets are able to modulate life-history treats in C. elegans model of Parkinson disease 150 150 SAN 2024 Annual Meeting

Disorders of the Nervous System
Author: Tania Veuthey | Email: tveuthey@uns.edu.ar


Tania Veuithey, Andreas Burkovski

Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB)-CONICET
Department Biologie, Friederich-Alexander-Universität Erlangen-Nürnberg

As life expectancy increase, age-related disorders, such us neurodegenerative diseases (ND), have become more prevalent. Moreover, treatments only attenuate some symptoms. Thus, new challenges emerge in order to understand molecular basis of these disorders. Lately, the gut-brain axis has gain attention and a close relation between gut microorganism and ND has been proposed. We evaluated the relevance of the microbiota assessing the impact of six non-pathogenic bacterial diets on life-history traits in C. elegans models of Parkinson disease (PD). In a first approach, we found 2 bacteria, Escherichia coli K12 and E. coli HB101, able to improve locomotion in liquid media, in in worms at adult day 4, versus E. coli OP50. Moreover, an age-dependent locomotion improvement, between larva-L4 and adult day 4, was observed in liquid media after feeding PD model´s worms with 4 different bacteria versus E. coli OP50. Similar results have been found tracking the movement of worms in solid media. We also observed an increase in the developmental timing of wild-type worms grown in 4 bacteria versus E. coli OP50, but more interesting was the accelerated developmental rate found in worms feed with E. coli BL21 (DE3). We are currently analyzing the changes in the proteoma of worms feed with different diets as well as aggregates numbers. Our results allowed us to identify bacteria with the ability to drive physiological outcomes and improve health status of C. elegans models of ND.

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