Cognition, Behavior, and Memory
Author: Melisa Riva Gargiulo | Email: melisa.gargiulo.080@unc.edu.ar
Melisa Riva Gargiulo1°2°, Ramiro Comas Mutis1°2°, Crhistian Bender1°2°, Lucas Pozzo Miller3°, Gastón Calfa1°2°
1° Instiruto de Faracología Experimental de Córdoba-IFEC
2° Departamento de Farmacología Otto Orsigher, Universidad Nacional de Córdoba
3° Department of Pediatrics & Human Development, Michigan State University
When a reminder is presented, an already consolidated memory can enter a labile state, followed by a new process of stabilization defined as destabilization/reconsolidation. In this sense, LTD could be critical for the weakening of synaptic connections. Previous studies have shown that such process is accompanied by the internalization of the glutamate receptor subunit GluA2 in the dorsal hippocampus (HD). However, it has been observed that emotionally relevant experiences, such as stress, prior to contextual fear conditioning generates resistance to destabilization/reconsolidation, with the consequent loss of the dynamic property of the fear memory. In the present study, we assessed if a stressful event influences the dynamic of LTD and GluA2 expression during destabilization/reconsolidation process in CA1. For this, we use previously stressed and conditioned C57BL/6 mice which were reactivated one day later in the same conditioning context. The animals were sacrificed before or 60 minutes after retrieval, CA1 DH was obtained for WB analysis and electrophysiology recordings. The preliminary findings showed that the stress exposure caused a higher excitatory tone in CA1 and an impediment in the internalization of GluA2 subunit.