V-015 | Dorsoventral and anteroposterior domains of the hypothalamus of two microchiroptera (Tadarida Brasiliensis and Myotis sp) living in South America.

Cellular and Molecular Neurobiology
Author: Franco Exequiel Lucero Arteaga | Email: francoluceroarteaga@hotmail.com

Franco Lucero Arteaga^1°2°, Ana Inés Portu^1°,Mariela Mondino^1°, Mónica Alejandra Boeris^1°, Valentina Heck^1°, Sofía Labegorra^1°, Ana Abrego Alvarez^1°, Ana Cristina Bratanich^3°, Bruno Ribeiro Do Couto^4°, José Luis Ferrán^4°

^1° Centro de producción de Animales de Experimentación, Facultad de Ciencias Veterinarias, Universidad Nacional de La Pampa.
^2° Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
^3° National Institute of Animal Production (INPA-CONICET), Faculty of Veterinary Sciences, University of Buenos Aires.
^4° Department of Human Anatomy and Psychobiology, School of Medicine, University of Murcia, Murcia, Spain. Institute of Biomedical Research of Murcia –IMIB, Virgen de la Arrixaca University Hospital, Murcia, Spain.

Microchiropteras or microbats are a diverse group of small bats that primarily feed on insects, though some species consume fruit, or blood. Microbats have a wide range of habitats, including forests, caves, and urban areas. They play an essential ecological role by controlling insect populations and pollinating plants. In our study, the insectivorous species Tadarida brasiliensis and Myotis spp. that inhabit in Argentina were selected. Our aim was to determine the hypothalamic neuronal derivatives in the peduncular and terminal regions of both microbats. Using the prosomeric model as a reference, the study was performed by immunoreactions with tyrosine hydroxylase (TH), Calbindin (CB), Calretinin (CR) NeuN, Adenosine-vasopressin (AVP), Oxytocin (OXT), Neurophysin I, II (NF1, NF2), and Melanin-concentrating-hormone (MCH). According to our analysis, alar plate TH positive neurons were detected periventricularly in the terminal and peduncular prosomeres in both species. In addition, AVP allowed to identify supraoptic (SO), suprachiasmatic and paraventricular (PV) nuclei; and OXT was detected in the SO and PV nuclei in both species. In the tuberal region (basal plate) TH positive cells were observed periventricularly. However, differences were observed in the Arquate and A13 nuclei between both species. Further studies will be required to determine possible size/composition differences with rodents. Grant: Fundación Seneca (21903/PI/22).